Methyl-directed repair of frameshift mutations in heteroduplex DNA.
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چکیده
منابع مشابه
Methyl-directed repair of frameshift mutations in heteroduplex DNA.
DNA heteroduplexes with single unpaired bases of the four different kinds were prepared by annealing separated strands of bacteriophage lambda DNA and used to transfect Escherichia coli. Genetic analysis of the progeny phages obtained from transfected bacteria indicates that the E. coli mismatch repair system can recognize and repair heteroduplexes with single unpaired bases--i.e., frameshift/w...
متن کاملMismatch repair in heteroduplex DNA.
DNA with base pair mismatches was prepared by annealing mixtures of genetically marked DNA from bacteriophage lambda. This heteroduplex DNA was used to transfect bacteria under conditions minimizing recombination. Genetic analysis of the progeny phages indicates that: (i) Mismatch repair occurs, usually giving rise to a DNA molecule with one chain with the genotype arising from repair and one p...
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We are interested in a sequence the more homogeneous as poss composition to compare its frequency with a less homogeneous one. First t codons composed by the repetition of the same nucleotide: AAA, CCC, GGG and TTT in Figure 1). If we look at the amino a acids that are associated also with frequency of 2 sequences undergoing differences due to the fact that the associated amino acids could be c...
متن کاملEffects of high levels of DNA adenine methylation on methyl-directed mismatch repair in Escherichia coli.
Two methods were used in an attempt to increase the efficiency and strand selectivity of methyl-directed mismatch repair of bacteriophage lambda heteroduplexes in E. coli. Previous studies of such repair used lambda DNA that was only partially methylated as the source of methylated chains. Also, transfection was carried out in methylating strains. Either of these factors might have been respons...
متن کاملMethyl-directed repair of DNA base-pair mismatches in vitro. 1983.
An assay has been developed that permits analysis of DNA mismatch repair in cell-free extracts of Escherichia coli. The method relies on repair of heteroduplex molecules of f1 R229 DNA, which contain a base-pair mismatch within the single EcoRI site of the molecule. As observed with mismatch heteroduplexes of lambda DNA [Pukkila, P. J., Peterson, J., Herman, G., Modrich, P. & Meselson, M. (1983...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1986
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.83.10.3395